| Strain Name |
C57BL/6-Il4tm2(IL4)Bcgen Il4ratm1(IL4RA)Bcgen/Bcgen
|
Common Name | B-hIL4/hIL4RA mice(C.B6) |
| Background | BALB/cCrSlcNifdc.C57BL/6JNifdc | Catalog number | 111889 |
|
Aliases |
BCGF-1, BCGF1, BSF-1, BSF1, IL-4 |
||
Protein expression analysis
H&E staining of asthma-like model in B-hIL4/hIL4RA mice(C.B6).Lung tissues were collected at the study endpoint. H&E staining results showed that the lung tissues from B-hIL4/hIL4RA mice(C.B6) exposed to PBS aerosols did not show any inflammation. OVA exposure resulted in a significant increase in peribronchial and perivascular inflammation in B-hIL4/hIL4RA mice(C.B6). A significant reduction in eosinophils infiltration was observed in mice treated with dupilumab (in house). (a) Mucus (b)Eosinophils
In vivo efficacy of anti-human IL4RA antibody with asthma mouse model
Measurement of enhanced pause (Penh) in mouse asthma model
Measurement of enhanced pause (Penh) by whole-body plethysmography. Airway responses following the exposure to increasing doses of methacholine (MCh) were measured for each mouse 24h after the final allergen or PBS exposure using the whole-body plethysmography. The y-axis represents the Penh absolute value. Increasing doses of methacholine were administered by aerosols. B-hIL4/hIL4RA mice(C.B6) (n = 6) exposed to OVA showed a significant increase in airway hyperreactivity to MCh when compared to B-hIL4/hIL4RA mice(C.B6) exposed to PBS inhalation (n=6) (*P<0.05, *** P<0.01). Penh values were significantly decreased in B-hIL4/hIL4RA mice(C.B6) treated with dupilumab (in house) when compared to B-hIL4/hIL4RA mice(C.B6) treated with hIgG4 (n = 6) after allergen exposure [MCh doses of 50mg/mL (#P<0.05)].
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