Application

• Function research of genes
• This Tcf21^iCreERT2 model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of Transcription factor 21 expressed during mouse embryogenesis specifically in mesodermally-derived cells that surround the epithelium of the developing gastrointestinal, genitourinary, and respiratory systems, and may be useful for studying mouse embryonic development. 

Expression

Expressed gene

Cre, cre recombinase, bacteriophage P1

Site of expression

Bladder adult, Lung adult, mouse embryos in mesenchymal cells 

Gene editing strategy

A iCreERT2-WPRE-pA sequence cassette was inserted downstream 5’UTR of the Tcf21 gene. This strain was maintained on a C57BL/6 genetic background.

Phenotype Analysis

Figure 2. Blocking SEV secretion from ECs delays AML progression. (A) The experimental design. (B–F) Survival curves of AML recipients with different genotypes. Cdh5-Cre;Vps33bfl/fl (B), Lepr-Cre;Vps33bfl/fl (C), Osx-CreER;Vps33bfl/fl (D), Pf4-Cre;Vps33bfl/fl (E), and Tcf21-CreER;Vps33bfl/fl (F) are shown (n = 5–8; **P < 0.01, ***P < 0.001, log-rank test). Experiments were conducted 3 times for validation.

They used 7 cell type–specific mouse Cre lines to conditionally knock out Vps33b in Cdh5+ or Tie2+ endothelial cells (ECs), Lepr+ BM perivascular cells, Osx+ osteoprogenitor cells, Pf4+ megakaryocytes, and Tcf21+ spleen stromal cells.                 

 (source: Huang D et al.,2021)

reference

1. Huang D, Sun G, Hao X, He X, Zheng Z, Chen C, Yu Z, Xie L, Ma S, Liu L, Zhou BO, Cheng H, Zheng J, Cheng T. ANGPTL2-containing small extracellular vesicles from vascular endothelial cells accelerate leukemia progression. J Clin Invest. 2021 Jan 4;131(1):e138986. [PubMed: 33108353]

2. Akama T, Chun TH. Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells. J Biol Chem. 2018 Apr 27;293(17):6603-6610. [PubMed: 29540474]